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HAINAN CHENGMEI HOSPITAL

Medical facility

Next-Generation Sequencing (NGS) System (Miseq)

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1.Device Technical Features and Performance

(1)Maximum Data Output per Sequencing Reaction: Up to 15 Gb (15 billion bases).

(2)Maximum Read Number per Sequencing Reaction: Up to 25 million sequences.

(3)Library Cloning and Amplification Principle: Bridge PCR.

(4)Multiplex SNP Analysis Capability: Simultaneous detection of over 589,000 amplicons in a single experiment (384 samples × 1536 amplicons per sample = 589,824 amplicons).

2.Clinical Application of the Device

The device supports rapid, cost-effective first-generation sequencing applications, such as sequencing of amplified products and clone validation. It is also suitable for next-generation genomic sequencing applications, including highly multiplexed PCR amplicon sequencing, small genome resequencing, de novo sequencing, small RNA sequencing, library quality control, and 16S bacterial metagenomic studies.

3.Department Using the Equipment

Pathology Department.